addgene toolkit 1000000156 Search Results


mochlo modular cloning chloroplast toolbox  (Addgene inc)
93
Addgene inc mochlo modular cloning chloroplast toolbox
Complete List of Golden Gate Toolkits Following the Common Syntax <xref ref-type= a " width="250" height="auto" />
Mochlo Modular Cloning Chloroplast Toolbox, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/addgene+toolkit+1000000156/MoChlo%3A+Modular+Cloning+Chloroplast+Toolbox+(Kit+%231000000156)/pmc09680027-13-0-6
Average 93 stars, based on 1 article reviews
mochlo modular cloning chloroplast toolbox - by Bioz Stars, 2026-10
93/100 stars
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destination vectors  (Addgene inc)
96
Addgene inc destination vectors
Proper design of restriction sites. For Golden Gate assembly to be successful, all part vectors must have inward-facing Type IIS sites, so that the enzyme recognition sequence is in the backbone (not the part); at the same time, <t>destination</t> vectors must have outward-facing sites, with the recognition sequence in the part (not the backbone). Also, the sequence of the fusion sites must be chosen so that parts that will be adjacent in the final assembly have complementary fusion sites. Clearly, the destination vector must have sites complementary to the first and the last fusion sites in the assembly, so that the entire construct will ligate into the destination vector.
Destination Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/addgene+toolkit+1000000156/Destination+Vector+(Plasmid+%23125754)/pmc09680027-13-18-6
Average 96 stars, based on 1 article reviews
destination vectors - by Bioz Stars, 2026-10
96/100 stars
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sylvestre marillonnet 215  (Addgene inc)
95
Addgene inc sylvestre marillonnet 215
Proper design of restriction sites. For Golden Gate assembly to be successful, all part vectors must have inward-facing Type IIS sites, so that the enzyme recognition sequence is in the backbone (not the part); at the same time, <t>destination</t> vectors must have outward-facing sites, with the recognition sequence in the part (not the backbone). Also, the sequence of the fusion sites must be chosen so that parts that will be adjacent in the final assembly have complementary fusion sites. Clearly, the destination vector must have sites complementary to the first and the last fusion sites in the assembly, so that the entire construct will ligate into the destination vector.
Sylvestre Marillonnet 215, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/addgene+toolkit+1000000156/MoClo+Toolkit+(Kit+%231000000044)/pm41533650-57-14-17
Average 95 stars, based on 1 article reviews
sylvestre marillonnet 215 - by Bioz Stars, 2026-10
95/100 stars
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mochlo 216 d ow  (Addgene inc)
94
Addgene inc mochlo 216 d ow
Proper design of restriction sites. For Golden Gate assembly to be successful, all part vectors must have inward-facing Type IIS sites, so that the enzyme recognition sequence is in the backbone (not the part); at the same time, <t>destination</t> vectors must have outward-facing sites, with the recognition sequence in the part (not the backbone). Also, the sequence of the fusion sites must be chosen so that parts that will be adjacent in the final assembly have complementary fusion sites. Clearly, the destination vector must have sites complementary to the first and the last fusion sites in the assembly, so that the entire construct will ligate into the destination vector.
Mochlo 216 D Ow, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/addgene+toolkit+1000000156/pCMV-eBE-S3+(Plasmid+%23101093)/pm41533650-57-31-80
Average 94 stars, based on 1 article reviews
mochlo 216 d ow - by Bioz Stars, 2026-10
94/100 stars
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fiag002 8425382  (Addgene inc)
94
Addgene inc fiag002 8425382
Proper design of restriction sites. For Golden Gate assembly to be successful, all part vectors must have inward-facing Type IIS sites, so that the enzyme recognition sequence is in the backbone (not the part); at the same time, <t>destination</t> vectors must have outward-facing sites, with the recognition sequence in the part (not the backbone). Also, the sequence of the fusion sites must be chosen so that parts that will be adjacent in the final assembly have complementary fusion sites. Clearly, the destination vector must have sites complementary to the first and the last fusion sites in the assembly, so that the entire construct will ligate into the destination vector.
Fiag002 8425382, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/addgene+toolkit+1000000156/pSLQ1852-5+pHR%3A+U6-SpsgCD95-3+CMV-EGFP+(Plasmid+%2384253)/pm41533650-57-41-80
Average 94 stars, based on 1 article reviews
fiag002 8425382 - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier

Image Search Results


Complete List of Golden Gate Toolkits Following the Common Syntax <xref ref-type= a " width="100%" height="100%">

Journal: ACS Synthetic Biology

Article Title: A User’s Guide to Golden Gate Cloning Methods and Standards

doi: 10.1021/acssynbio.2c00355

Figure Lengend Snippet: Complete List of Golden Gate Toolkits Following the Common Syntax a

Article Snippet: MoChlo: Modular Cloning Chloroplast Toolbox , Addgene toolkit 1000000156 , ( ) , Chloroplast-specific genetic modules. Also includes destination vectors for tobacco and potato. , KanR , BsaI.

Techniques: Marker, Transformation Assay, Expressing, Ubiquitin Proteomics, Selection, Plasmid Preparation, Infection, Cloning, CRISPR, Bacteria

Alternative Golden Gate Methods That Do Not Follow the Common Syntax <xref ref-type= a " width="100%" height="100%">

Journal: ACS Synthetic Biology

Article Title: A User’s Guide to Golden Gate Cloning Methods and Standards

doi: 10.1021/acssynbio.2c00355

Figure Lengend Snippet: Alternative Golden Gate Methods That Do Not Follow the Common Syntax a

Article Snippet: MoChlo: Modular Cloning Chloroplast Toolbox , Addgene toolkit 1000000156 , ( ) , Chloroplast-specific genetic modules. Also includes destination vectors for tobacco and potato. , KanR , BsaI.

Techniques: Selection, Expressing, Marker

Proper design of restriction sites. For Golden Gate assembly to be successful, all part vectors must have inward-facing Type IIS sites, so that the enzyme recognition sequence is in the backbone (not the part); at the same time, destination vectors must have outward-facing sites, with the recognition sequence in the part (not the backbone). Also, the sequence of the fusion sites must be chosen so that parts that will be adjacent in the final assembly have complementary fusion sites. Clearly, the destination vector must have sites complementary to the first and the last fusion sites in the assembly, so that the entire construct will ligate into the destination vector.

Journal: ACS Synthetic Biology

Article Title: A User’s Guide to Golden Gate Cloning Methods and Standards

doi: 10.1021/acssynbio.2c00355

Figure Lengend Snippet: Proper design of restriction sites. For Golden Gate assembly to be successful, all part vectors must have inward-facing Type IIS sites, so that the enzyme recognition sequence is in the backbone (not the part); at the same time, destination vectors must have outward-facing sites, with the recognition sequence in the part (not the backbone). Also, the sequence of the fusion sites must be chosen so that parts that will be adjacent in the final assembly have complementary fusion sites. Clearly, the destination vector must have sites complementary to the first and the last fusion sites in the assembly, so that the entire construct will ligate into the destination vector.

Article Snippet: MoChlo: Modular Cloning Chloroplast Toolbox , Addgene toolkit 1000000156 , ( ) , Chloroplast-specific genetic modules. Also includes destination vectors for tobacco and potato. , KanR , BsaI.

Techniques: Sequencing, Plasmid Preparation, Construct

Destination vectors as acceptor backbones. The destination vector of a Golden Gate assembly provides the origin or replication and selection marker of the final construct (dashed box). Note that the fusion sites of the destination vector (<>) appear in the opposite orientation compared to those of the part vectors (><). The cargo of the destination vector (gray CDS) is a dropout marker, which is lost during the assembly. Therefore, the final construct can be isolated by combining positive selection for the backbone marker (typically antibiotic resistance), and negative screening against the dropout marker (usually a visual marker such as lacZα ).

Journal: ACS Synthetic Biology

Article Title: A User’s Guide to Golden Gate Cloning Methods and Standards

doi: 10.1021/acssynbio.2c00355

Figure Lengend Snippet: Destination vectors as acceptor backbones. The destination vector of a Golden Gate assembly provides the origin or replication and selection marker of the final construct (dashed box). Note that the fusion sites of the destination vector (<>) appear in the opposite orientation compared to those of the part vectors (><). The cargo of the destination vector (gray CDS) is a dropout marker, which is lost during the assembly. Therefore, the final construct can be isolated by combining positive selection for the backbone marker (typically antibiotic resistance), and negative screening against the dropout marker (usually a visual marker such as lacZα ).

Article Snippet: MoChlo: Modular Cloning Chloroplast Toolbox , Addgene toolkit 1000000156 , ( ) , Chloroplast-specific genetic modules. Also includes destination vectors for tobacco and potato. , KanR , BsaI.

Techniques: Plasmid Preparation, Selection, Marker, Construct, Isolation

Multilevel hierarchical assembly. If a destination vector contains inward sites in addition to outward sites, the inward sites will be retained in the final assembly and can then be used to excise the product as if it was a part. In this example, both destination vectors have the same outward-facing sites (blue<>yellow), but different inward-facing sites (respectively blue><green and green><yellow). Consequently, the intermediate products are also part vectors, and share a complementary fusion site (green). The intermediate parts can be assembled in a new destination vector, resulting in a final product that is also a part vector. Note that each assembly step uses a different endonuclease (here BsaI and BsmBI) and resistance marker (here ampicillin and kanamycin).

Journal: ACS Synthetic Biology

Article Title: A User’s Guide to Golden Gate Cloning Methods and Standards

doi: 10.1021/acssynbio.2c00355

Figure Lengend Snippet: Multilevel hierarchical assembly. If a destination vector contains inward sites in addition to outward sites, the inward sites will be retained in the final assembly and can then be used to excise the product as if it was a part. In this example, both destination vectors have the same outward-facing sites (blue<>yellow), but different inward-facing sites (respectively blue>

Article Snippet: MoChlo: Modular Cloning Chloroplast Toolbox , Addgene toolkit 1000000156 , ( ) , Chloroplast-specific genetic modules. Also includes destination vectors for tobacco and potato. , KanR , BsaI.

Techniques: Plasmid Preparation, Marker

Complete List of Golden Gate Toolkits Following the Common Syntax <xref ref-type= a " width="100%" height="100%">

Journal: ACS Synthetic Biology

Article Title: A User’s Guide to Golden Gate Cloning Methods and Standards

doi: 10.1021/acssynbio.2c00355

Figure Lengend Snippet: Complete List of Golden Gate Toolkits Following the Common Syntax a

Article Snippet: MoChlo: Modular Cloning Chloroplast Toolbox , Addgene toolkit 1000000156 , ( ) , Chloroplast-specific genetic modules. Also includes destination vectors for tobacco and potato. , KanR , BsaI.

Techniques: Marker, Transformation Assay, Expressing, Ubiquitin Proteomics, Selection, Plasmid Preparation, Infection, Cloning, CRISPR, Bacteria